6T7E
PII-like protein CutA from Nostoc sp. PCC7120 in complex with MES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 87.073, 87.073, 152.994 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.249 - 2.450 |
| Rwork | 0.222 |
| R-free | 0.27130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6t76 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.243 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049 2013/06/30) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.250 | 2.510 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.092 | |
| Number of reflections | 25325 | 3985 |
| <I/σ(I)> | 16.98 | |
| Completeness [%] | 99.8 | |
| Redundancy | 8.7 | |
| CC(1/2) | 0.999 | 0.842 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1 MES pH 6.5, 1.2 M Potassium/Sodium tartrate |
