6T76
PII-like protein CutA from Nostoc sp. PCC 7120 in apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-16 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.133, 90.694, 93.707 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.850 - 1.900 |
| Rwork | 0.199 |
| R-free | 0.22580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v6h |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.801 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049 2013/06/30) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.854 | 2.010 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.099 | |
| Number of reflections | 42415 | 6637 |
| <I/σ(I)> | 13.4 | |
| Completeness [%] | 99.7 | 97.9 |
| Redundancy | 6.55 | 6.34 |
| CC(1/2) | 0.999 | 0.749 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1 M Bis-Tris pH 6.5, 2 M Ammonium sulphate. |
