6T74
New antiparallel dimer of aureochrome 1a LOV domain mutants from Phaeodactylum tricornutum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-26 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 100.746, 100.746, 120.570 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.520 - 1.900 |
Rwork | 0.154 |
R-free | 0.17480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5a8b |
RMSD bond length | 0.011 |
RMSD bond angle | 1.652 |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.520 | 1.968 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.012 | 0.292 |
Rmeas | 0.017 | 0.413 |
Rpim | 0.012 | 0.292 |
Number of reflections | 49476 | 4862 |
<I/σ(I)> | 24.51 | 2.39 |
Completeness [%] | 100.0 | 100 |
Redundancy | 2 | 2 |
CC(1/2) | 1.000 | 0.838 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291.15 | in 1.1 M sodium malonate, 0.1 M HEPES pH 7.0, 0.5 % Jeffamine ED-2003 under dark conditions |