6T6G
Bacteroides salyersiae GH164 beta-mannosidase in complex with noeuromycin
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-24 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.916 |
| Spacegroup name | P 1 |
| Unit cell lengths | 69.754, 105.096, 170.544 |
| Unit cell angles | 92.60, 97.21, 105.10 |
Refinement procedure
| Resolution | 90.191 - 2.060 |
| Rwork | 0.212 |
| R-free | 0.23990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6t5o |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.613 |
| Data reduction software | DIALS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.130 | 2.100 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Number of reflections | 280992 | 13781 |
| <I/σ(I)> | 5.2 | |
| Completeness [%] | 98.3 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.986 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1 M ammonium tartrate pH 7.0, 13 % PEG 3350 |
