6T6E

Crystal Structure of the C-terminal domain of the HIV-1 Integrase (PNL4-3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-20
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0
Spacegroup name	P 63
Unit cell lengths	49.472, 49.472, 39.811
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.844 - 1.302
R-factor	0.1803
Rwork	0.176
R-free	0.21930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qmc 1ihv
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_3311)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.844	1.350
High resolution limit [Å]	1.300	1.300
Rmerge	0.059	4.151
Number of reflections	13357	1227
<I/σ(I)>	254580
Completeness [%]	99.1
Redundancy	18.6
CC(1/2)	1.000	0.249

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Tris pH 7, 0.8 M potassium sodium tartrate and 0.2 M lithium sulfate