6T6C

Complex with chitin oligomer of C-type lysozyme from the upper gastrointestinal tract of Opisthocomus hoatzin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	CCD
Collection date	2012-09-16
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	37.051, 55.008, 65.829
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.247 - 1.250
Rwork	0.108
R-free	0.13430
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	6t5s
RMSD bond length	0.018
RMSD bond angle	2.191
Data reduction software	MOSFLM
Data scaling software	Aimless
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.247	1.270
High resolution limit [Å]	1.250	1.250
Rmerge	0.105	0.882
Number of reflections	38043	1893
<I/σ(I)>	13.4
Completeness [%]	99.9
Redundancy	8
CC(1/2)	0.998	0.535

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.5	293	0.1 M Citric acid pH 3.5, 3.0 M Sodium chloride, Hampton research INDEX screen no 7.