6T5S
Apo form of C-type lysozyme from the upper gastrointestinal tract of Opisthocomus hoatzin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.973, 54.895, 65.319 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.060 - 1.500 |
| Rwork | 0.116 |
| R-free | 0.16570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gft |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.744 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.060 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.074 | 0.535 |
| Number of reflections | 21950 | 1065 |
| <I/σ(I)> | 12.3 | 1.9 |
| Completeness [%] | 99.8 | |
| Redundancy | 5 | |
| CC(1/2) | 0.998 | 0.646 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 150 nl of protein was mixed with 150 nl of mother liquor solution. Crystals grew in 0.1 M MIB system (malonic acid, imidazole, boric acid) pH 4.0 and 25% Peg 3350 |
