6T5L

MYO-1 from Myroides odoratimimus. Environmental metallo-beta-lactamases exhibit high enzymatic activity under zinc deprivation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-22
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 65
Unit cell lengths	144.684, 144.684, 53.306
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.530 - 2.170
R-factor	0.219
Rwork	0.217
R-free	0.25260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1znb
RMSD bond length	0.014
RMSD bond angle	1.301
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.240
High resolution limit [Å]	2.170	2.170
Rmerge	0.058	0.052
Rpim	0.039	0.754
Number of reflections	33621	2941
<I/σ(I)>	11
Completeness [%]	99.7	99.8
Redundancy	3.5
CC(1/2)	0.998	0.354

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	32-36% PEG4000, 0.2 ammonium sulphate