6T5F
Human 14-3-3 sigma fused to the StARD1 peptide including phosphoserine-195
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-30 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97950 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 77.160, 74.950, 104.380 |
Unit cell angles | 90.00, 97.98, 90.00 |
Refinement procedure
Resolution | 51.690 - 2.630 |
R-factor | 0.219 |
Rwork | 0.217 |
R-free | 0.26000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5om0 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.120 |
Data reduction software | XDS |
Data scaling software | xia2 |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.510 | 2.720 |
High resolution limit [Å] | 2.630 | 2.630 |
Rmerge | 0.150 | 1.380 |
Rmeas | 0.170 | |
Number of reflections | 35345 | 3496 |
<I/σ(I)> | 5.4 | 1.1 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 4 | 4 |
CC(1/2) | 0.990 | 0.480 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 283 | 20% w/v polyethylene glycol 3350 |