6T5F
Human 14-3-3 sigma fused to the StARD1 peptide including phosphoserine-195
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-30 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.160, 74.950, 104.380 |
| Unit cell angles | 90.00, 97.98, 90.00 |
Refinement procedure
| Resolution | 51.690 - 2.630 |
| R-factor | 0.219 |
| Rwork | 0.217 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5om0 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.510 | 2.720 |
| High resolution limit [Å] | 2.630 | 2.630 |
| Rmerge | 0.150 | 1.380 |
| Rmeas | 0.170 | |
| Number of reflections | 35345 | 3496 |
| <I/σ(I)> | 5.4 | 1.1 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 4 | 4 |
| CC(1/2) | 0.990 | 0.480 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 283 | 20% w/v polyethylene glycol 3350 |
