6T5A

Crystal structure of herpes simplex virus 1 pUL7:pUL51 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	P 1 21 1
Unit cell lengths	79.510, 106.300, 106.010
Unit cell angles	90.00, 92.05, 90.00

Refinement procedure

Resolution	26.570 - 1.830
R-factor	0.195
Rwork	0.194
R-free	0.22000
Structure solution method	MAD
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	105.900	1.860
High resolution limit [Å]	1.830	1.830
Rmerge	0.120	3.197
Rpim	0.051	1.367
Number of reflections	154984	7654
<I/σ(I)>	6.8	0.5
Completeness [%]	100.0	99.9
Redundancy	6.5	6.5
CC(1/2)	0.996	0.321

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	289.15	pUL7:pUL51 complex was crystallized in sitting or hanging drops by mixing 1 uL of 5.3 mg/mL protein with 0.5 uL of 0.5 M benzamidine hydrochloride and 1 uL of reservoir solution containing 0.15 mM sodium citrate pH 5.5, 12% (v/v) 2-methyl-2,4-pentanediol, 0.1 M NaCl and equilibrating against 200 uL reservoirs at 16 C for at least one week.