6T5A
Crystal structure of herpes simplex virus 1 pUL7:pUL51 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-01 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 79.510, 106.300, 106.010 |
Unit cell angles | 90.00, 92.05, 90.00 |
Refinement procedure
Resolution | 26.570 - 1.830 |
R-factor | 0.195 |
Rwork | 0.194 |
R-free | 0.22000 |
Structure solution method | MAD |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | CRANK2 |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 105.900 | 1.860 |
High resolution limit [Å] | 1.830 | 1.830 |
Rmerge | 0.120 | 3.197 |
Rpim | 0.051 | 1.367 |
Number of reflections | 154984 | 7654 |
<I/σ(I)> | 6.8 | 0.5 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 6.5 | 6.5 |
CC(1/2) | 0.996 | 0.321 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289.15 | pUL7:pUL51 complex was crystallized in sitting or hanging drops by mixing 1 uL of 5.3 mg/mL protein with 0.5 uL of 0.5 M benzamidine hydrochloride and 1 uL of reservoir solution containing 0.15 mM sodium citrate pH 5.5, 12% (v/v) 2-methyl-2,4-pentanediol, 0.1 M NaCl and equilibrating against 200 uL reservoirs at 16 C for at least one week. |