6T4C
Bovine enterovirus F3 in complex with glutathione
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-12 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 342.750, 348.160, 351.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.980 - 1.800 |
| Rwork | 0.205 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5osn |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.980 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.394 | |
| Rpim | 0.111 | |
| Number of reflections | 1902973 | 94026 |
| <I/σ(I)> | 6.2 | 0.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 13.4 | 7.8 |
| CC(1/2) | 0.987 | 0.200 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 1.5 M Ammonium Sulfate and 0.1 M Tris at pH 8.5 |
