6T3X
Crystal structure of the truncated human cytomegalovirus pUL50-pUL53 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-28 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.918400 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.270, 82.570, 63.660 |
Unit cell angles | 90.00, 95.10, 90.00 |
Refinement procedure
Resolution | 41.280 - 1.480 |
R-factor | 0.1944 |
Rwork | 0.192 |
R-free | 0.22490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5d5n |
RMSD bond length | 0.005 |
RMSD bond angle | 0.784 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MLPHARE |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.290 | 1.520 |
High resolution limit [Å] | 1.480 | 1.480 |
Rmeas | 0.090 | 0.929 |
Number of reflections | 63159 | 4515 |
<I/σ(I)> | 8.9 | 1.59 |
Completeness [%] | 98.7 | 96.3 |
Redundancy | 4 | 3.9 |
CC(1/2) | 0.993 | 0.592 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | The protein was dissolved in a buffer consisting of 50 mM TrisHCl, 150 mM NaCl, pH 7.5 and concentrated to values between 10-15 mg/ml. Diffraction quality crystals of pUL50::pUL53 were obtained at 4 degree C with 20% PEG 4000, 10% propanol, 100 mM HEPES, pH 7.5 as a reservoir solution. |