6T3H

Structure of the Rap conjugation gene regulator of the plasmid pLS20 in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97927
Spacegroup name	P 21 21 2
Unit cell lengths	111.790, 174.487, 49.879
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.231 - 3.039
R-factor	0.2042
Rwork	0.201
R-free	0.25930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ulq
RMSD bond length	0.004
RMSD bond angle	0.734
Data reduction software	XDS (Jun 1, 2017)
Data scaling software	autoPROC (staraniso 1.0.4)
Phasing software	PHASER (2.6.1)
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	87.200	3.092
High resolution limit [Å]	3.039	3.039
Rmerge	0.064	0.900
Rmeas	0.075	1.041
Rpim	0.044	0.615
Number of reflections	18645	797
<I/σ(I)>	14.8	1.4
Completeness [%]	95.3	82.5
Redundancy	4.6	4.5
CC(1/2)	0.607	0.607

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	290	10% Peg 6000, 0.1M Hepes pH 7. Protein concentration 10mg/mL