6T3H
Structure of the Rap conjugation gene regulator of the plasmid pLS20 in apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97927 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 111.790, 174.487, 49.879 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.231 - 3.039 |
R-factor | 0.2042 |
Rwork | 0.201 |
R-free | 0.25930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ulq |
RMSD bond length | 0.004 |
RMSD bond angle | 0.734 |
Data reduction software | XDS (Jun 1, 2017) |
Data scaling software | autoPROC (staraniso 1.0.4) |
Phasing software | PHASER (2.6.1) |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 87.200 | 3.092 |
High resolution limit [Å] | 3.039 | 3.039 |
Rmerge | 0.064 | 0.900 |
Rmeas | 0.075 | 1.041 |
Rpim | 0.044 | 0.615 |
Number of reflections | 18645 | 797 |
<I/σ(I)> | 14.8 | 1.4 |
Completeness [%] | 95.3 | 82.5 |
Redundancy | 4.6 | 4.5 |
CC(1/2) | 0.607 | 0.607 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 10% Peg 6000, 0.1M Hepes pH 7. Protein concentration 10mg/mL |