6T2Z
Streptavidin variants harbouring an artificial organocatalyst based cofactor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-16 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 57.378, 84.536, 98.988 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.810 - 1.350 |
| R-factor | 0.165 |
| Rwork | 0.164 |
| R-free | 0.18280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6t1e |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.777 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.810 | 1.398 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Number of reflections | 52425 | 4879 |
| <I/σ(I)> | 9.27 | |
| Completeness [%] | 98.7 | |
| Redundancy | 6.6 | |
| CC(1/2) | 0.966 | 0.349 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.6M Na2HPO4 0.4M K2HPO4 0.1M Sodium citrate phosphate pH 4.2 |
