6T2Y
Streptavidin variants harbouring an artificial organocatalyst based cofactor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 57.515, 84.449, 98.665 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.540 - 1.800 |
Rwork | 0.193 |
R-free | 0.23980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6t1e |
RMSD bond length | 0.004 |
RMSD bond angle | 0.765 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.540 | 1.864 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 41969 | 4149 |
<I/σ(I)> | 4.8 | |
Completeness [%] | 97.7 | |
Redundancy | 7.6 | |
CC(1/2) | 0.985 | 0.229 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M di-Sodium hydrogen phosphate 20% PEG 3350 |