6T2U
Cryo-EM structure of the RecBCD in complex with Chi-minus2 substrate
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.6 |
| Number of Particle | 754150 |
| Software | Gautomatch,RELION,PHENIX,EPU,Gctf |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
