6T2R
Prominent members of the human gut microbiota express endo-acting O-glycanases to initiate mucin breakdown
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 46.928, 87.135, 156.193 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.570 - 2.100 |
| Rwork | 0.197 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3iln |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.738 |
| Data reduction software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.570 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 19202 | 1554 |
| <I/σ(I)> | 1.6 | 1.6 |
| Completeness [%] | 100.0 | |
| Redundancy | 7.1 | 7.2 |
| CC(1/2) | 0.995 | 0.743 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 1 M ammonium sulfate |
