6T2R

Prominent members of the human gut microbiota express endo-acting O-glycanases to initiate mucin breakdown

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	C 2 2 21
Unit cell lengths	46.928, 87.135, 156.193
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.570 - 2.100
Rwork	0.197
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3iln
RMSD bond length	0.011
RMSD bond angle	1.738
Data reduction software	xia2
Phasing software	PHASER
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.570	2.160
High resolution limit [Å]	2.100	2.100
Number of reflections	19202	1554
<I/σ(I)>	1.6	1.6
Completeness [%]	100.0
Redundancy	7.1	7.2
CC(1/2)	0.995	0.743

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	1 M ammonium sulfate