6T2O
Prominent members of the human gut microbiota express endo-acting O-glycanases to initiate mucin breakdown
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9159 |
| Spacegroup name | P 41 |
| Unit cell lengths | 82.646, 82.646, 121.489 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 82.646 - 2.050 |
| Rwork | 0.199 |
| R-free | 0.24020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3iln |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.525 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.650 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 51105 | 3974 |
| <I/σ(I)> | 6.3 | 1.6 |
| Completeness [%] | 100.0 | |
| Redundancy | 7.4 | |
| CC(1/2) | 0.986 | 0.958 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 200 mM sodium malonate pH 7.0, 20% PEG 3350 |
