6T2N
Prominent members of the human gut microbiota express endo-acting O-glycanases to initiate mucin breakdown
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-01-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 87.465, 96.128, 128.756 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.716 - 2.700 |
Rwork | 0.233 |
R-free | 0.28110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5wut |
RMSD bond length | 0.014 |
RMSD bond angle | 2.202 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.900 | 2.830 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 33933 | 4096 |
<I/σ(I)> | 7.7 | 1 |
Completeness [%] | 99.7 | |
Redundancy | 6.4 | |
CC(1/2) | 0.991 | 0.485 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 1.6 M Tri sodium citrate pH 6.5 |