6T2L
Streptavidin variants harbouring an artificial organocatalyst based cofactor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-08 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 57.215, 57.215, 174.866 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.720 - 1.000 |
R-factor | 0.1235 |
Rwork | 0.123 |
R-free | 0.13420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6t1e |
RMSD bond length | 0.008 |
RMSD bond angle | 1.102 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.720 | 1.036 |
High resolution limit [Å] | 1.000 | 1.000 |
Number of reflections | 78168 | 7610 |
<I/σ(I)> | 9.49 | |
Completeness [%] | 99.5 | |
Redundancy | 8.5 | |
CC(1/2) | 0.998 | 0.356 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 273 | 0.17M Na-acetate, 0.085M TRIS-HCl, pH 8.5, 25.5% PEG 4000, 15% glycerol |