6T2A
Mopeia Virus Exonuclease domain partially complexed with Manganese
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 93.15 |
Detector technology | PIXEL |
Collection date | 2018-06-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9720 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 45.597, 37.969, 137.379 |
Unit cell angles | 90.00, 93.31, 90.00 |
Refinement procedure
Resolution | 43.969 - 2.000 |
R-factor | 0.2345 |
Rwork | 0.232 |
R-free | 0.26680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6sx8 |
Data reduction software | XDS (20190315) |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 137.151 | 1.788 |
High resolution limit [Å] | 1.758 | 1.758 |
Rmerge | 0.029 | 0.361 |
Rpim | 0.019 | 0.238 |
Number of reflections | 44701 | 2273 |
<I/σ(I)> | 17.1 | 2.1 |
Completeness [%] | 94.3 | |
Redundancy | 3 | |
CC(1/2) | 0.999 | 0.965 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 0.1 M MES pH 6.5 and 25 % (M/W) PEG 8000) |