6T28

Crystal structure of human calmodulin-dependent protein kinase 1D (CAMK1D) bound to compound 19 (CS640)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-19
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.91840
Spacegroup name	I 1 2 1
Unit cell lengths	57.640, 45.620, 108.949
Unit cell angles	90.00, 104.44, 90.00

Refinement procedure

Resolution	31.780 - 1.550
Rwork	0.157
R-free	0.18290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jc6
RMSD bond length	0.008
RMSD bond angle	1.436
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.800	1.580
High resolution limit [Å]	1.550	1.550
Number of reflections	34389	1829
<I/σ(I)>	10.9
Completeness [%]	86.2
Redundancy	4
CC(1/2)	0.996	0.806

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.9	293	0,1M NaCit, pH 5.9 2,05M AmmSO4 0,1M Na/K tartrate