6T1U
Cytochrome P450 reductase from Candida tropicalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2018-08-06 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.91587 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.289, 67.184, 143.466 |
| Unit cell angles | 90.00, 91.50, 90.00 |
Refinement procedure
| Resolution | 52.270 - 1.500 |
| R-factor | 0.1736 |
| Rwork | 0.172 |
| R-free | 0.19940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bn4 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.683 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.17) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.180 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.081 | 0.757 |
| Rmeas | 0.097 | 0.902 |
| Rpim | 0.052 | 0.486 |
| Number of reflections | 220306 | 10825 |
| <I/σ(I)> | 7.6 | |
| Completeness [%] | 97.8 | 97 |
| Redundancy | 3.4 | 3.4 |
| CC(1/2) | 0.994 | 0.665 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 289 | 4% Tacsimate pH 5.0, 12% PEG 3350 |
