6T1K
Streptavidin variants harbouring an artificial organocatalyst based cofactor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 57.275, 57.275, 175.019 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.750 - 1.200 |
R-factor | 0.1572 |
Rwork | 0.157 |
R-free | 0.17090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6t1e |
RMSD bond length | 0.008 |
RMSD bond angle | 1.151 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.750 | 1.240 |
High resolution limit [Å] | 1.200 | 1.200 |
Number of reflections | 46054 | 4461 |
<I/σ(I)> | 10.47 | |
Completeness [%] | 99.8 | |
Redundancy | 10.7 | |
CC(1/2) | 0.999 | 0.301 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | CHES 0.1 M pH 9.5 30% PEG 3000 |