6T0Y
Crystal structure of YlmD from Geobacillus stearothermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-09 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97628 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.780, 48.636, 134.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.435 - 1.200 |
R-factor | 0.1492 |
Rwork | 0.148 |
R-free | 0.16680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1t8h |
Data reduction software | XDS (11) |
Data scaling software | Aimless (0.7.3) |
Phasing software | PHASER (1.16-3549) |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.610 | 1.250 |
High resolution limit [Å] | 1.190 | 1.190 |
Rmerge | 0.049 | 3.571 |
Rmeas | 0.053 | 3.889 |
Rpim | 0.021 | 1.521 |
Number of reflections | 88850 | 12741 |
<I/σ(I)> | 12.4 | |
Completeness [%] | 99.9 | 6.58 |
Redundancy | 6.5 | 6.3 |
CC(1/2) | 0.999 | 0.377 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 25% PEG 6000, 0.5 M NaCl, 0.1 M HEPES pH 7.5 |