6T03
Crystal structure of YTHDC1 with fragment 16 (DHU_DC1_017)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-03 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.854, 103.672, 42.555 |
| Unit cell angles | 90.00, 105.73, 90.00 |
Refinement procedure
| Resolution | 40.960 - 1.500 |
| R-factor | 0.1749 |
| Rwork | 0.174 |
| R-free | 0.19460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r3h |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.885 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.000 | 1.620 |
| High resolution limit [Å] | 1.320 | 1.500 |
| Number of reflections | 123265 | 19862 |
| <I/σ(I)> | 13.74 | |
| Completeness [%] | 79.9 | |
| Redundancy | 1.74 | |
| CC(1/2) | 0.998 | 0.966 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 25% PEG3350, 0.2M Ammonium Sulphate, 0.1M bis-tris, pH 6.5 |
