6SYM
Crystal structure of Escherichia coli MsrB (reduced form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-25 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.28 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.390, 56.270, 85.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.042 - 1.630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | model obtained from Phyre2 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.14-3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 7.290 | 1.670 |
High resolution limit [Å] | 1.630 | 7.290 | 1.630 |
Rmerge | 0.139 | 0.069 | 1.215 |
Rmeas | 0.150 | 0.073 | 1.643 |
Number of reflections | 61381 | 694 | 4140 |
<I/σ(I)> | 8.47 | 26.34 | 0.69 |
Completeness [%] | 98.7 | 100 | 89.4 |
Redundancy | 6.776 | 8.441 | 1.677 |
CC(1/2) | 0.996 | 0.993 | 0.305 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277.15 | 0.1M sodium cacodylate pH 6, 30% PEG 2K MME, 0.2M MgCl2 |