6SYJ
Crystal structure of a ProM2 containing triple-helical collagen peptide.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.8266 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 26.080, 22.850, 53.430 |
| Unit cell angles | 90.00, 103.83, 90.00 |
Refinement procedure
| Resolution | 25.260 - 0.810 |
| Rwork | 0.128 |
| R-free | 0.13630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ah9 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.907 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17rc5-3630) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.260 | 0.839 |
| High resolution limit [Å] | 0.810 | 0.810 |
| Rmerge | 0.038 | 0.817 |
| Rmeas | 0.042 | 0.975 |
| Rpim | 0.016 | 0.521 |
| Number of reflections | 57795 | 3409 |
| <I/σ(I)> | 17.74 | 1.17 |
| Completeness [%] | 92.3 | 55.12 |
| Redundancy | 6.1 | 3.3 |
| CC(1/2) | 1.000 | 0.605 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 1.1 M (NH4)2HPO4, 0.1 M Tris |
