6SYH
Wolinella succinogenes L-asparaginase mutant V23Q,K24T in P 22121
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-25 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 60.619, 79.959, 108.469 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.190 - 1.500 |
| R-factor | 0.1422 |
| Rwork | 0.140 |
| R-free | 0.18720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6rud |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.706 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.060 | 0.160 |
| Number of reflections | 79211 | 5972 |
| <I/σ(I)> | 2.01 | |
| Completeness [%] | 98.3 | |
| Redundancy | 4.72 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | COUNTER-DIFFUSION | 293 | SA 1.4 |
