6SYG

Crystal structure of the Cyclic Nucleotide-Binding Homology Domain of the human KCNH2 channel

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-12
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.972
Spacegroup name	C 2 2 21
Unit cell lengths	57.493, 90.649, 61.111
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.550 - 1.500
R-factor	0.1706
Rwork	0.170
R-free	0.18400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4llo
RMSD bond length	0.008
RMSD bond angle	1.058
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.580	1.590
High resolution limit [Å]	1.500	1.500
Rmeas	0.114	0.811
Number of reflections	25875	4087
<I/σ(I)>	13.91
Completeness [%]	99.7
Redundancy	11.5
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	20% w/v PEG 3350, 2% v/v Tacsimate, 0.1M Bis-Tris, pH 6.8