6SY8
Mopeia Virus Exonuclease domain fully depleted of Manganese un ALD compound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 93.15 |
Detector technology | PIXEL |
Collection date | 2018-05-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97857 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 45.853, 38.290, 137.315 |
Unit cell angles | 90.00, 92.63, 90.00 |
Refinement procedure
Resolution | 68.580 - 2.080 |
R-factor | 0.25 |
Rwork | 0.248 |
R-free | 0.28300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6sx8 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.030 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.2) |
Phasing software | PHASER (2.8.2) |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 137.170 | 137.170 | 2.200 |
High resolution limit [Å] | 2.080 | 6.590 | 2.080 |
Rmerge | 0.121 | 0.053 | 0.993 |
Rmeas | 0.135 | 0.059 | 1.096 |
Rpim | 0.058 | 0.027 | 0.455 |
Total number of observations | 151996 | 4327 | 22552 |
Number of reflections | 28863 | 974 | 4153 |
<I/σ(I)> | 10.6 | 25.3 | 2.4 |
Completeness [%] | 99.6 | 99 | 99.4 |
Redundancy | 5.3 | 4.4 | 5.4 |
CC(1/2) | 0.996 | 0.996 | 0.942 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293.15 | 0.1 M MES 25 % (M/W) PEG 8000) 3mM Mn |