6SY6
TetR in complex with the TetR-binding RNA-aptamer K2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-17 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 96.564, 96.564, 119.971 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 16.110 - 2.900 |
R-factor | 0.2497 |
Rwork | 0.248 |
R-free | 0.28820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ns7 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.697 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 16.110 | 3.003 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.814 | 5.662 |
Rmeas | 0.835 | 5.799 |
Rpim | 0.184 | 1.248 |
Number of reflections | 14683 | 1443 |
<I/σ(I)> | 4.6 | 0.47 |
Completeness [%] | 99.2 | 100 |
Redundancy | 20.3 | 21.4 |
CC(1/2) | 0.975 | 0.241 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 15 % (v/v) pentaerythritol propoxylate (5/4 PO/OH), 0.2 M NaCl, pH 6.0, 0.1 M MES-NaOH |