6SY0
Structure of the Plasmodium falciparum SIP2 DNA-binding AP2 tandem repeat in complex with two SPE2 half-sites
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-08 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.91741 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 80.350, 43.720, 153.710 |
| Unit cell angles | 90.00, 92.17, 90.00 |
Refinement procedure
| Resolution | 43.931 - 3.102 |
| R-factor | 0.2758 |
| Rwork | 0.274 |
| R-free | 0.31840 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 153.600 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.084 | 1.088 |
| Rmeas | 0.091 | 1.164 |
| Number of reflections | 19852 | 1912 |
| <I/σ(I)> | 15.24 | 2.26 |
| Completeness [%] | 97.0 | 100 |
| Redundancy | 7.6 | 8 |
| CC(1/2) | 1.000 | 0.927 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 277 | 0.16 M calcium acetate 100 mM cacodylic acid 6.5% w/v PEG 8000 20% v/v glycerol |
