6SXW
Crystal structure of the first RRM domain of human Zinc finger protein 638 (ZNF638)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-09-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.92 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 57.435, 57.435, 46.619 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 18.800 - 2.751 |
R-factor | 0.2649 |
Rwork | 0.260 |
R-free | 0.31310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cq1 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.800 | 2.900 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.050 | 1.666 |
Rpim | 0.018 | 0.569 |
Number of reflections | 2464 | 343 |
<I/σ(I)> | 20.9 | 1.5 |
Completeness [%] | 99.7 | 100 |
Redundancy | 9.4 | 9.5 |
CC(1/2) | 1.000 | 0.724 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1M tris pH 8.5 -- 2.6M ammonium sulfate |