6SXC
Crystal structure of the Voltage-Gated Sodium Channel NavMs (F208L) in complex with 4-hydroxytamoxifen (2.5 Angstrom resolution)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9762 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 109.125, 109.125, 210.596 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.410 - 2.500 |
R-factor | 0.231 |
Rwork | 0.230 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hvx |
RMSD bond length | 0.010 |
RMSD bond angle | 1.040 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.410 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 22430 | 2478 |
<I/σ(I)> | 19.5 | 2.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 20 | 15 |
CC(1/2) | 1.000 | 0.963 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.1 M NaCl, 0.1M Calcium chloride dehydrate, 0.1 M Hepes buffer, pH 7, 30 v/v polyethylene glycol (PEG) 400 |