6SX0
Specific dsRNA recognition by wild type H7N1 NS1 RNA-binding domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-08 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 0.98010 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.785, 60.397, 87.396 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.259 - 1.750 |
R-factor | 0.1731 |
Rwork | 0.171 |
R-free | 0.20270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6sw8 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.3) |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.260 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.052 | 1.546 |
Rmeas | 0.057 | 1.675 |
Rpim | 0.022 | 0.640 |
Number of reflections | 27291 | 1481 |
<I/σ(I)> | 16.7 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.5 | 6.8 |
CC(1/2) | 0.999 | 0.571 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 293 | 100mM CHES, 30% PEG400 |