6SWH
Crystal structure of the ternary complex between the type 1 pilus proteins FimC, FimI and FimA from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 61 |
| Unit cell lengths | 198.350, 198.350, 87.560 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.891 - 2.800 |
| R-factor | 0.1713 |
| Rwork | 0.171 |
| R-free | 0.20700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sqb |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15rc3_3435) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 22.000 | 22.000 | 3.000 |
| High resolution limit [Å] | 2.800 | 21.000 | 2.800 |
| Rmerge | 0.066 | 0.024 | 0.576 |
| Rmeas | 0.072 | 0.027 | 0.643 |
| Number of reflections | 48258 | 18 | 8945 |
| <I/σ(I)> | 21.27 | 63.06 | 3.07 |
| Completeness [%] | 99.3 | 13.5 | 99.2 |
| Redundancy | 5.851 | 5.5 | 5.137 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.8 M sodium formate, 100 mM Tris/acetic acid pH 8.5, 16% PEG 4000 (w/v) |
