6SWF
Selenomethionine derivative of the REC domain of AraT, a response regulator from Geobacillus stearothermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.131, 32.892, 78.656 |
| Unit cell angles | 90.00, 97.29, 90.00 |
Refinement procedure
| Resolution | 39.010 - 2.601 |
| R-factor | 0.2434 |
| Rwork | 0.235 |
| R-free | 0.32530 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.135 | 0.525 |
| Number of reflections | 7778 | 765 |
| <I/σ(I)> | 13.3 | 2.7 |
| Completeness [%] | 99.3 | 98.2 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 41% PPG 425 and 0.1 M Bis-Tris pH 6 |
