6SWB

The REC domain of AraT, a response regulator from Geobacillus stearothermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	32.499, 41.918, 43.981
Unit cell angles	83.46, 89.98, 71.11

Refinement procedure

Resolution	31.162 - 2.259
R-factor	0.1762
Rwork	0.168
R-free	0.25540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6swf
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.400
High resolution limit [Å]	2.259	2.259
Rmerge	0.060	0.265
Number of reflections	9678	1203
<I/σ(I)>	17.3	5.2
Completeness [%]	94.5
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	41% PPG 425 and 0.1 M Bis-Tris pH 6