6SW8
Crystal structure of the NS1 (H7N1) RNA-binding domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-29 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 0.98009 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 83.827, 83.827, 68.527 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.832 - 1.933 |
R-factor | 0.1763 |
Rwork | 0.175 |
R-free | 0.19320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5h5n |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.832 | 1.970 |
High resolution limit [Å] | 1.930 | 1.930 |
Rmerge | 0.078 | 1.090 |
Number of reflections | 21170 | 21170 |
<I/σ(I)> | 15.9 | 1.6 |
Completeness [%] | 100.0 | |
Redundancy | 11.3 | |
CC(1/2) | 0.999 | 0.704 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.12M (Diethylene glycol, Triethylene glycol, Tetraethylene glycol, Pentaethylene glycol) + 0.1M (Sodium HEPES, MOPS) + 37.5% (MPD, PEG 1000, PEG 3350) |