6SW4

The structure of AraP, an arabinose binding protein from Geobacillus stearothermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2017-12-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	46.790, 95.600, 128.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.984 - 1.845
R-factor	0.2362
Rwork	0.234
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3g1w
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.960
High resolution limit [Å]	1.840	1.840
Rmerge	0.065	1.357
Number of reflections	50563	7925
<I/σ(I)>	22.8
Completeness [%]	99.6
Redundancy	14.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	23% PEG 8K, 0.1 M acetate buffer pH 4.5