6SVS

Crystal Structure of U:A-U-rich RNA triple helix with 11 consecutive base triples

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	54.716, 78.114, 84.018
Unit cell angles	90.00, 104.20, 90.00

Refinement procedure

Resolution	40.700 - 2.500
R-factor	0.1689
Rwork	0.167
R-free	0.21340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4plx
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.700	40.700	2.700
High resolution limit [Å]	2.500	8.340	2.500
Rmerge	0.039	0.025	1.470
Rmeas	0.045	0.029	1.660
Number of reflections	13040	648	664
<I/σ(I)>	17.9	40.9	1.1
Completeness [%]	54.4	95.7	13
Redundancy	3.9
CC(1/2)	1.000	1.000	0.400

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	The crystallization drop included 1.5 ul of MALAT1_th11 RNA and 1 ul reservoir solution containing 50 mM sodium cacodylate pH 6.0-6.3, 200 mM calcium acetate, and 2.5 M sodium chloride. Crystals were cryoprotected by stepwise addition of crystallization solution supplemented with glycerol until the final concentration of 20% glycerol was reached.