6SVK

human Myeloid-derived growth factor (MYDGF)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-19
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.99999
Spacegroup name	P 31 2 1
Unit cell lengths	48.677, 48.677, 177.846
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.155 - 1.604
R-factor	0.219
Rwork	0.218
R-free	0.24700
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.070
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELXD
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.155	1.707
High resolution limit [Å]	1.604	1.604
Rmerge	0.215	2.299
Number of reflections	28207	1409
<I/σ(I)>	11.9	1.3
Completeness [%]	85.1	25.6
Redundancy	19.1	15
CC(1/2)	0.998	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.4	277.15	48% M1K3350 (12% w/v PEG 1000, 12% w/v PEG 3350, and 12% v/v MPD, original solution Molecular Dimensions), 1,25% NPS (3.75 mM sodium nitrate, 3.75 mM sodium phosphate dibasic, and 3.75 mM ammonium sulfate; original solution MolecularDimensions), 0.1M MMT buffer (pH 6.4) (0.02 M DL-malic acid, 0.04 M MES monohydrate, 0.04 M Tris)