6SVF
Crystal structure of the P235GK mutant of ArgBP from T. maritima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-09-28 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.720, 52.964, 95.686 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.600 |
| R-factor | 0.173 |
| Rwork | 0.171 |
| R-free | 0.21750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ggp |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.032 |
| Data reduction software | EVAL15 |
| Data scaling software | EVAL15 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.657 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmeas | 0.080 | 0.345 |
| Rpim | 0.037 | 0.227 |
| Number of reflections | 30630 | 2798 |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 97.5 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | protein concentration in the range 6-10 mg/ml in a solution containing 2.0 M ammonium sulfate and 5 % (v/v) 2-propanol |
