6SUP
Crystal Structure of TcdB2-TccC3-Cdc42
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-04 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.979580 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 96.350, 156.550, 179.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.170 - 2.000 |
Rwork | 0.287 |
R-free | 0.32000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4o9x |
Data reduction software | XDS (VERSION Mar 15, 2019 BUILT=20190315) |
Data scaling software | XSCALE (VERSION Mar 15, 2019 BUILT=20190315) |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.170 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 182401 | 18014 |
<I/σ(I)> | 13.81 | 1.46 |
Completeness [%] | 99.9 | |
Redundancy | 22 | |
CC(1/2) | 0.998 | 0.688 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293.15 | 0.1 M sodium chloride, 0.1 M magnesium chloride, 0.1 M tri-sodium citrate pH 5.5, 12 % PEG 4000 Seeding: 0.1 M magnesium chloride, 0.1 M tri-sodium acetate pH 4.6, 12 % PEG 6000 |