6SUJ
Mutations in SsgB correlate to longitudinal cell division during sporulation of Streptomyces coelicolor
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.975 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.933, 93.219, 96.241 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.167 - 3.200 |
Rwork | 0.236 |
R-free | 0.29350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6slc |
RMSD bond length | 0.007 |
RMSD bond angle | 1.467 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.900 | 2.610 |
High resolution limit [Å] | 2.560 | 2.560 |
Rmerge | 0.446 | 3.642 |
Rmeas | 0.466 | 3.894 |
Rpim | 0.134 | 1.344 |
Number of reflections | 20149 | 20149 |
<I/σ(I)> | 4.8 | 1.1 |
Completeness [%] | 99.6 | 91.6 |
Redundancy | 12.1 | 8.4 |
CC(1/2) | 0.991 | 0.560 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 291 | 0.2 M Potassium sodium tartrate, 0.1 M Bis-tris prop (pH 7.5), 20% PEG3350 |