6SU9
Crystal structure of Plasmodium falciparum PdxK with ligands AMP-PNP and PL
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-08 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.703, 62.004, 93.712 |
Unit cell angles | 90.00, 94.99, 90.00 |
Refinement procedure
Resolution | 19.327 - 2.150 |
R-factor | 0.2126 |
Rwork | 0.209 |
R-free | 0.27190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rfu |
RMSD bond length | 0.008 |
RMSD bond angle | 0.952 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.330 | 2.227 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.042 | 0.042 |
Number of reflections | 32284 | 3159 |
<I/σ(I)> | 2 | |
Completeness [%] | 97.9 | 97.17 |
Redundancy | 2.55 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | 0.1 M HEPES pH 7.75, 0.2 M CaCl2, 31%(v/v) PEG400, 5%(v/v) glycerol |