6SU8

Highly thermostable endoglucanase Cel7B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9802
Spacegroup name	C 2 2 21
Unit cell lengths	113.367, 263.212, 197.824
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.480
Rwork	0.170
R-free	0.19000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2a39
RMSD bond length	0.008
RMSD bond angle	1.108
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.566
High resolution limit [Å]	2.477	2.477
Rmeas	0.133	0.900
Rpim	0.048	0.326
Number of reflections	104697	10160
<I/σ(I)>	8.86	1.9
Completeness [%]	99.7	97.8
Redundancy	7.3	7.4
CC(1/2)	0.993	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	Sodium Malonate 2.4M