6SU8
Highly thermostable endoglucanase Cel7B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9802 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 113.367, 263.212, 197.824 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.480 |
Rwork | 0.170 |
R-free | 0.19000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2a39 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.108 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 2.566 |
High resolution limit [Å] | 2.477 | 2.477 |
Rmeas | 0.133 | 0.900 |
Rpim | 0.048 | 0.326 |
Number of reflections | 104697 | 10160 |
<I/σ(I)> | 8.86 | 1.9 |
Completeness [%] | 99.7 | 97.8 |
Redundancy | 7.3 | 7.4 |
CC(1/2) | 0.993 | 0.580 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | Sodium Malonate 2.4M |