6SU6
Complex between a UDP-glucosyltransferase from Polygonum tinctorium capable of glucosylating indoxyl and UDP-glucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.976484 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 121.240, 174.170, 48.510 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 57.250 - 2.400 |
R-factor | 0.2322 |
Rwork | 0.231 |
R-free | 0.26320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5nlm |
RMSD bond length | 0.003 |
RMSD bond angle | 0.585 |
Data reduction software | xia2 (0.4) |
Data scaling software | xia2 (0.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.250 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.106 | 1.154 |
Number of reflections | 40705 | 3679 |
<I/σ(I)> | 17.13 | 1.98 |
Completeness [%] | 98.8 | 90.96 |
Redundancy | 10.4 | 8.6 |
CC(1/2) | 0.999 | 0.657 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.2M MgCl2, 0.1M HEPES pH 7.5, 25% PEG 3350 |