6SU2
Trypanosoma congolense pyruvate kinase in complex with citrate and glycerol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2016-09-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.99986 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 112.770, 182.460, 244.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.740 - 3.000 |
| R-factor | 0.252 |
| Rwork | 0.250 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.190 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.740 | 3.107 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.193 | 2.616 |
| Rmeas | 0.201 | 2.718 |
| Rpim | 0.054 | 0.730 |
| Number of reflections | 50876 | 5019 |
| <I/σ(I)> | 12.18 | 0.88 |
| Completeness [%] | 99.9 | |
| Redundancy | 13.6 | |
| CC(1/2) | 0.998 | 0.347 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 50 mM magnesium acetate, 225 mM sodium acetate, 10% PEG 8000, 2.5 mM F16BP |
